- API Development & Manufacture
- Peptide & Protein Technology
- rapidd™
- Analytical
- Solid State Services
- Isotope Chemistry
- Biocatalysis / selectAZyme™
- FLEXYTE™ Fluorescence Lifetime Assays
- Animal Free Chemokines
Keyword
DUB Assays
FLEXYTE™ assays harness the power and potential of fluorescence lifetime technology for screening and profiling applications:
- Assays available for a range of drug target classes:
- Robust and reliable performance
- Homogeneous and antibody free
- Minimises interference from problem compounds
- Applicable to fragment based screening
FLEXYTE™ Isopeptide DUB assays
- FLEXYTE™ FLT DUB assay platform uses full length isopeptide-linked Ub substrates to determine DUB activity
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Ub is generated with the 9AA fluorescence lifetime reporter site-specifically attached to the C-terminus of the protein via a lysine isopeptide bond
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A known FLT modulator, M, is incorporated at a selected site within the Ub domain modulating the lifetime of 9AA in the substrate
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Cleavage of the physiologically-relevant Ub substrate by DUBs is directly reported through a change in the fluorescence lifetime
- USP catalysed cleavage of the C-terminal isopeptide bond of Ub is reported through increases in the measured FLT
- IC50 values for known inhibitors in good agreement with those reported in the literature
- Excellent Z’-factors demonstrate the assay’s suitability for HTS applications
