Deiminases

Deiminases

FLEXYTE Assays Peptidylarginine Deiminases (PAD)

PAD Assay Principle

  • Peptide substrate labelled with proprietary long lifetime 9-aminoacridine (9AA) fluorophore and incorporating an aromatic moiety which reduces the FLT of 9AA
  • Deiminase catalysed citrullination of the arginine residue prevents cleavage by the protease, trypsin, thereby maintaining modulation of the FLT of 9AA
  • Hence, deiminase activity is reported by a decrease in the measured FLT of the assay

PAD4 Assay

  • A  FLEXYTE™ deiminase assay has been developed for PAD4, which is implicated in autoimmune diseases such as rheumatoid arthritis
  • High assay sensitivity ensures low nM or pM enzyme requirements
  • High Z’-factors confirm assay is suitable for HTS
  • The protease protection approach to assaying deiminases has recently been extended to PAD2
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