Kinases

Kinases

Almac’s FLEXYTE™ Ser / Thr protein kinase platform uses three generic peptide substrates to configure assays for a broad panel of kinases and the use of tailored substrates for specific kinases as required. This homogeneous, antibody-free approach uses a phosphoserine or phosphothreonine chelating agent to induce a change in the fluorescence lifetime of the 9AA label. We have now configured this technology for assaying Ser / Thr phosphatases using bespoke 9AA-labelled phosphorylated peptide substrates. In addition, Almac now offer the utility of 9AA-labelled peptide substrates for configuring fluorescence lifetime tyrosine kinase assays.

These homogeneous, real-time assays can be configured for a broad range of tyrosine kinases using appropriate 9AA-labelled peptide substrates.

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