Almac

• Simply clone protein of interest as an intein fusion
• Express fusion protein in host of choice
• Affinity purify fusion protein from lysates using commercially available reagents
• Treat fusion protein with aqueous hydrazine
• Enzyme free liberation of target protein (folded) with unique C-terminal hydrazide functionality
  • No extra amino acids appended to target sequence
  • Recombinant C-terminal hydrazide protein can be produced in folded form
• Treat Recombinant hydrazide protein with pyruvoyl-PEG reagent(s) under aqueous conditions.
• Site-specific mono-PEGylated folded protein generated in high yield.

Compatible with disulphide bond containing proteins

This technology is covered by WO 2005014620.