Hydrolase Enzyme Screening Panel: HESK-4800
The selectAZyme
TMHESK-4800 panel consists of 48 commercially available hydrolase enzymes. Hydrolases, in particular lipases, dominate the field of biocatalysis due to their commercial availability, large number available for screening, a lack of need for expensive or demanding co-factors, versatility for synthetic or hydrolytic usage, and suitability for both aqueous and solvent-based reactions. From an industrial perspective they often admirably meet the essential criteria for “industrial readiness”, such as availability, performance (turnover, selectivity, volume efficiency) and freedom from intellectual property issues. These enzymes accept a very broad range of substrates and exhibit good to excellent selectivity.
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Carbonyl Reductase Enzyme Screening Panel: CESK-5000
Almac has developed the selectAZyme
TM CESK-5000 panel which consists of 50 proprietary carbonyl reductases (CREDs) that show good selectivity and activity for the reduction of prochiral ketones to chiral alcohols with high optical purity. Asymmetric synthesis of chiral alcohols has the obvious advantage of 100% theoretical yield when compared to resolution technology. These enzymes accept a very broad range of substrates
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Aldehyde Reductase Enzyme Screening Panel: AESK-1600
The selectAZyme
TMAESK-1600 panel consists of 16 aldehyde reductases (AREDs) that are useful for the synthesis of primary alcohols by reduction of aldehydes. They can also be used to selectively reduce an aldehyde in the presence of a ketone, or in the presence of other aldehydes. These enzymes exhibit a broad substrate range.
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Transaminase Enzyme Screening Panel: TESK-3600
The selectAZyme
TMTESK-3600 panel consists of 36 transaminase (TAm) enzymes that can be used for the synthesis of chiral amines from prochiral ketones or to resolve racemic amines.
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Nitrile Manipulator Enzyme Screening Panel: NESK-2500
The selectAZyme
TMNESK-2500 panel consists of 9 nitrile hydratase (NHase) enzymes and 16 nitrilase enzymes. NHases are a class of metalloenzyme that can be used for the mild hydrolysis of nitriles to give the primary amide as the direct biotransformation product. In contrast, nitrilases hydrolyse nitriles directly to the carboxylic acid. Given the harsh conditions of acid, base or high temperatures that are often required to perform such a reaction chemically, a major benefit of nitrilases is their ability to perform nitrile hydrolysis in neutral buffer solutions at ambient temperatures. Like many other enzymes, NHases and nitrilases can effect bioresolution of racemic substrates, often with high stereoselectivity. These enzymes exhibit a broad substrate range.
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Epoxide Hydrolase Enzyme Screening Panel: EHESK-500
The selectAZyme
TM EHESK-500 panel consists of 5 epoxide hydrolase enzymes that can be used for the resolution of racemic epoxides by selective ring opening in aqueous media to provide optically active
anti diols and optically active epoxides.
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Phenylalanine Dehydrogenase Enzyme Screening Panel: PDESK-500
The selectAZyme
TMPDESK-500 panel consists of 5 phenylalanine dehydrogenase enzymes that can be used for the synthesis of L-amino acids by reductive amination of 2-oxo acids.
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P450 Monooxygenase Enzyme Screening Panel: OxESK-1000
The selectAZyme
TM OxESK-1000 panel consists of 10 P
450monooxygenase enzymes that can be used for selective oxidation of unactivated carbons to produce alcohols and phenols, selective epoxidation of olefins, as well as N- and O-dealkylations. These enzymes exhibit a remarkable substrate promiscuity.
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Ene Reductase Enzyme Screening Panel: ERESK-1300
The selectAZyme
TMERESK-1300 panel consists of 13 ene reductase (ERED) enzymes that can be used for the asymmetric reduction of electron-deficient olefins. This allows the formation of up to two new chiral centres from pro-chiral olefins. They also allow regioselective reduction in a molecule containing more than one alkene moiety.
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Hyperactivated Hydrolase Enzyme Screening Panel: HAHESK-1000
Hyperactivation leads to dramatic cost reductions in any enzymatic process as the observed reaction rate enhancement is generally 20x to 100x greater than that obtained with the untreated enzyme. The selectAZyme
TMHAHESK-1000 panel consists of 10 second generation hydrolase enzymes which offer superior performance when compared to standard commercially available enzymes. These enzymes can tolerate far higher substrate loadings while maintaining high E values.
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Miscellaneous Enzymes
Almac’s selectAZyme
TM platform also offers a variety of miscellaneous enzymes including glucose dehydrogenase (GDH) for the
in situ recycling of NAD(P)H cofactor during reactions with CREDs, AREDs, transaminases, phenylalanine dehydrogenases, ene reductases and P
450 monooxygenases, as well as lactate dehydrogenase (LDH) for the conversion of pyruvate to lactate during transamination reactions and dihydrofolate reductase for the conversion of imines to amines.
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R&D quantities and commercial supply of the biocatalysts are available upon request.
