Carbonyl Reductase

Carbonyl reductases (CREDs) are enzymes useful for enantiomeric resolutions, chemo- and regioselective reductions/oxidations, and synthesis of chiral alcohols from ketones, ketoacids, and ketoesters. They also catalyse the reduction of a number of aldehydes.

These enzymes are stereoselective and exhibit a very broad substrate range.

• CREDs stereoselectively reduce carbonyl groups.
• CREDs stereoselectively oxidize secondary alcohols.
• CREDs exhibit high diastereoselectivity. A reduction can stereoselectively fix two chiral centers in a single step.
• In the reduction of 2-substituted-3-ketoesters, a rapid racemization can occur prior to reduction, dynamically resolving a racemic starting material into a single diastereomerically pure product with two chiral carbons.

The CRED catalysed reduction of the carbonyl moiety requires cofactor (NADH or NADPH) regeneration and is carried out with either isopropyl alcohol or with glucose dehydrogenase as shown below:

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