Custom Peptide Synthesis for Research Use


Custom Peptide Synthesis For ResearchSpecialising in High Quality Peptides

Our custom peptide synthesis offering is based on decades of trading and hundreds of working years gaining valuable experience in the field. During this time, we have produced in excess of 9,000 peptides on a custom synthesis basis and we have become experts in the production of long peptides and complex peptide structures such as folded proteins and peptide conjugates.

We have a proven track record in high quality supply, while providing excellent customer service through the balance of technical expertise and project management.

Custom Synthesis Capabilities

Peptides are produced by Fmoc / tBu solid phase synthesis and can be manufactured on the scale of mgs to 10s of grams. Most projects are completed in an efficient high throughput basis using parallel synthesis equipment, with a short lead time of less than 4 weeks.

Our entire range of standard peptide modifications are available per your request, including labelling with biotin, fluorescein or other fluorophores, or the formation of disulfide bridges. Unnatural building blocks, such as phosphorylated, acylated or methylated amino acids, are routinely incorporated into the peptide sequence. Additionally, we have a license from Life TechnologiesTM that enables us to incorporate the highly desirable AlexaFluor® dyes into synthetic peptides.

Technically Challenging Peptides

Long Peptides (Click to Read More)

We routinely synthesise peptides in excess of 100 amino acids in length. The longest peptide we have manufactured by linear solid phase synthesis was made from 276 amino acids.

Folded Proteins (Click to Read More)

We have broad experience in synthesising peptides and short proteins containing multiple disulfide bridges, ensuring that the molecule has the desired biological function. Chemokines, Defensins, Conotoxins, and Complement Proteins are all examples of products that we have successfully produced.

Cyclised peptides (Click to Read More)

Cyclised peptides enable conformational and chemical stability. Head-to-tail cyclisation, lactam bridge formation, “stapled” peptides through olefin metathesis and directed disulfide bridges, are all types of the cyclisation methods we employ.

Specialist Catalogue Peptides

We provide complex peptides as catalogue products for basic biological research.

We have our well established catalogue of chemokine products for use in wide-ranging research. More recently we have established a range of products for epigenetics research such as histones and ubiquitylated peptides (see tabs below in the MORE DETAILS section).

These products are highly specialist, and many are unique to Almac, offering physiologically relevant reagents for research.

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More Details

Chemokines & Complement Proteins
Modified Histones
Ubiquitylated Peptides
Peptide Conjugation

Chemokines & Complement Proteins

Chemokines

Extensive Number of Human and Mouse Chemokines Available

From research tools to therapeutics, chemokines have a wide and complex range of applications in the biochemical research field. We provide a range of over 60 human chemokines in their native and site-specific labelled forms.

We employ proprietary skills in long-chain peptide synthesis to chemically synthesise chemokines. As a result, high purity products are obtained, ensuring the precise amino acid sequence is produced endotoxin free. This selective manufacturing process is highly amenable to the manufacture of labelled chemokines, ensuring that a single label is incorporated at a specific site in the sequence.

We make available site-specifically labelled chemokines with biotin and AlexaFluor 647®.

All of our products are available in various pack sizes and can be purchased from our online shop.

All of our products are available in various pack sizes:

button (1)

 

Please see our chemokine FAQs for more details

If you would like further information, please contact us at chemokines@almacgroup.com

Download our Comprehensive Chemokine Services catalogue


Modified Histones

Modified Histones

Regulating Regular and Disease-Specific Cellular Processes

Our proprietary platform technologies for the chemical synthesis of proteins enable the provision of pure, full-length natural histones, containing a wide variety of post-translational modifications, at any specified site(s) within the protein.

Our synthetic modified histones correspond exactly to the sequences of the natural modified histones, containing no amino acid replacements or residue analogues. They can be used in a variety of applications, such as substrates for specific histone modification enzymes, protein binding assays, and the generation of chromatin preparations.

Currently available in on our Histone catalogue are:

  • H3 native
  • H3 K9me
  • H3 K9ac K14ac
  • H3 K9me2
  • H3 K4ac K9ac K14ac K18ac K23ac
  • H3 K9me3
  • H3 S10p K14ac
  • H3 K4me3
  • H3 S10p
  • H3 R2me

If you would like further information, please contact us at histones@almacgroup.com


Ubiquitylated Peptides

Ubiquitylated Peptides

We provide several reagents that incorporate full-length ubiquitin site-specifically onto a peptide. Products are entirely synthetic, ensuring precision over the labelling position and product purity. Linkage is via the native isopeptide-bond, using high yielding methodology. These reagents are physiologically relevant Ub substrates for DUB assays, and fluorescent analogues are available for drug screening and profiling applications.

We have several off-the shelf-reagents available:

  • AUB-001 Ac-PTEN(Ub)-NH2
  • AUB-002 Ac-H2B(Ub)-NH2
  • AUB-101 5’-TAMRA-K(Ub)-NH2

We can also help in reagent design, or to synthesise your custom requests.

Enquiries and Orders

If you would like further information, please contact us at peptides@almacgroup.com


Peptide Conjugation

Almac has extensive experience in the conjugation of peptides to a range of carrier proteins including keyhole limpet hemocyanin (KLH), Ovalbumin, Bovine Serum Albumin (BSA), and Human Serum Albumin (HSA).  Conjugation can be achieved using a wide variety of linkages, exploiting cysteine or lysine residues in the target peptide. The target peptide is manufactured to a high purity and excess peptide and linker are removed post-conjugation using size exclusion chromatography to produce a clean product.  Characterisation of the level of incorporation of the peptide on the surface of the carrier protein can be completed using high resolution mass spectrometry techniques.

Conjugation to Small Molecules

Working with fluorophores, chelating agents and therapeutics

We have proven expertise in the conjugation of peptides to a wide variety of small molecules, which can be designed and manufactured using a wide variety of linkages.

Our license with Life Technologies allows us access to conjugate synthetic peptides to a wide range of highly effective fluorophores, such as AlexaFluor®. Additionally, we have expertise in the synthesis of imaging agent precursors, such as DOTA and NOTA.

We have developed our own in-house technology to allow for the post-folding labelling of small proteins. This methodology, which utilises a masked cysteine moiety, can be used to introduce a fluorophore or PEG molecule bearing maleimide functionality into the product after the disulfide bridges have been formed. This methodology has been applied to a number of chemokine, complement protein and conotoxin targets.

Conjugation to Small Molecules

Peptide-Peptide Conjugation

Combining our extensive experience with unique technologies

Peptide-peptide conjugates can be designed and manufactured using a wide variety of linkages, including thiol-thiol, thioether, oximes and amine-thiol crosslinks. Each peptide component is manufactured to a high purity and the final conjugated product is purified by RP-HPLC to generate a clean, well-characterised product. The most recent addition to our peptide conjugation technology is the ability to site-specifically ubiquitylate peptides through an isopeptide linkage. This is completely unique to Almac and provides access to valuable tools in biological research.

Site-Specific Modification of Recombinant Proteins

Robust technology for total control over the conjugation process

We have developed a generic and robust technology for the site-specific attachment of small molecules, large polymers and peptides to various proteins. This methodology, which produces a targeted, site-specific ligation at the C-terminus, allows for total control over the conjugation process.

Our robust technology has many advantages: it’s high yielding and uses low ligand or label equivalents, the process is completed under aqueous conditions, it is compatible with disulfide bond containing proteins, it allows us to retain biological activity and, by using a single modification site, we can ensure a homogeneous product is produced. This technology is widely applicable to a range of labels and modifications and it has the potential to improve upon the native protein – for example, by modifying the protein to extend its half-life while maintaining biological activity.

Site-specific modification of Recombinant Proteins

 

 




More Details

Chemokines & Complement Proteins
Modified Histones
Ubiquitylated Peptides
Peptide Conjugation

Chemokines & Complement Proteins

Chemokines

Extensive Number of Human and Mouse Chemokines Available

From research tools to therapeutics, chemokines have a wide and complex range of applications in the biochemical research field. We provide a range of over 60 human chemokines in their native and site-specific labelled forms.

We employ proprietary skills in long-chain peptide synthesis to chemically synthesise chemokines. As a result, high purity products are obtained, ensuring the precise amino acid sequence is produced endotoxin free. This selective manufacturing process is highly amenable to the manufacture of labelled chemokines, ensuring that a single label is incorporated at a specific site in the sequence.

We make available site-specifically labelled chemokines with biotin and AlexaFluor 647®.

All of our products are available in various pack sizes and can be purchased from our online shop.

All of our products are available in various pack sizes:

button (1)

 

Please see our chemokine FAQs for more details

If you would like further information, please contact us at chemokines@almacgroup.com

Download our Comprehensive Chemokine Services catalogue


Modified Histones

Modified Histones

Regulating Regular and Disease-Specific Cellular Processes

Our proprietary platform technologies for the chemical synthesis of proteins enable the provision of pure, full-length natural histones, containing a wide variety of post-translational modifications, at any specified site(s) within the protein.

Our synthetic modified histones correspond exactly to the sequences of the natural modified histones, containing no amino acid replacements or residue analogues. They can be used in a variety of applications, such as substrates for specific histone modification enzymes, protein binding assays, and the generation of chromatin preparations.

Currently available in on our Histone catalogue are:

  • H3 native
  • H3 K9me
  • H3 K9ac K14ac
  • H3 K9me2
  • H3 K4ac K9ac K14ac K18ac K23ac
  • H3 K9me3
  • H3 S10p K14ac
  • H3 K4me3
  • H3 S10p
  • H3 R2me

If you would like further information, please contact us at histones@almacgroup.com


Ubiquitylated Peptides

Ubiquitylated Peptides

We provide several reagents that incorporate full-length ubiquitin site-specifically onto a peptide. Products are entirely synthetic, ensuring precision over the labelling position and product purity. Linkage is via the native isopeptide-bond, using high yielding methodology. These reagents are physiologically relevant Ub substrates for DUB assays, and fluorescent analogues are available for drug screening and profiling applications.

We have several off-the shelf-reagents available:

  • AUB-001 Ac-PTEN(Ub)-NH2
  • AUB-002 Ac-H2B(Ub)-NH2
  • AUB-101 5’-TAMRA-K(Ub)-NH2

We can also help in reagent design, or to synthesise your custom requests.

Enquiries and Orders

If you would like further information, please contact us at peptides@almacgroup.com


Peptide Conjugation

Almac has extensive experience in the conjugation of peptides to a range of carrier proteins including keyhole limpet hemocyanin (KLH), Ovalbumin, Bovine Serum Albumin (BSA), and Human Serum Albumin (HSA).  Conjugation can be achieved using a wide variety of linkages, exploiting cysteine or lysine residues in the target peptide. The target peptide is manufactured to a high purity and excess peptide and linker are removed post-conjugation using size exclusion chromatography to produce a clean product.  Characterisation of the level of incorporation of the peptide on the surface of the carrier protein can be completed using high resolution mass spectrometry techniques.

Conjugation to Small Molecules

Working with fluorophores, chelating agents and therapeutics

We have proven expertise in the conjugation of peptides to a wide variety of small molecules, which can be designed and manufactured using a wide variety of linkages.

Our license with Life Technologies allows us access to conjugate synthetic peptides to a wide range of highly effective fluorophores, such as AlexaFluor®. Additionally, we have expertise in the synthesis of imaging agent precursors, such as DOTA and NOTA.

We have developed our own in-house technology to allow for the post-folding labelling of small proteins. This methodology, which utilises a masked cysteine moiety, can be used to introduce a fluorophore or PEG molecule bearing maleimide functionality into the product after the disulfide bridges have been formed. This methodology has been applied to a number of chemokine, complement protein and conotoxin targets.

Conjugation to Small Molecules

Peptide-Peptide Conjugation

Combining our extensive experience with unique technologies

Peptide-peptide conjugates can be designed and manufactured using a wide variety of linkages, including thiol-thiol, thioether, oximes and amine-thiol crosslinks. Each peptide component is manufactured to a high purity and the final conjugated product is purified by RP-HPLC to generate a clean, well-characterised product. The most recent addition to our peptide conjugation technology is the ability to site-specifically ubiquitylate peptides through an isopeptide linkage. This is completely unique to Almac and provides access to valuable tools in biological research.

Site-Specific Modification of Recombinant Proteins

Robust technology for total control over the conjugation process

We have developed a generic and robust technology for the site-specific attachment of small molecules, large polymers and peptides to various proteins. This methodology, which produces a targeted, site-specific ligation at the C-terminus, allows for total control over the conjugation process.

Our robust technology has many advantages: it’s high yielding and uses low ligand or label equivalents, the process is completed under aqueous conditions, it is compatible with disulfide bond containing proteins, it allows us to retain biological activity and, by using a single modification site, we can ensure a homogeneous product is produced. This technology is widely applicable to a range of labels and modifications and it has the potential to improve upon the native protein – for example, by modifying the protein to extend its half-life while maintaining biological activity.

Site-specific modification of Recombinant Proteins

 

 



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