{"id":50894,"date":"2020-02-03T15:33:31","date_gmt":"2020-02-03T15:33:31","guid":{"rendered":"https:\/\/www.almacgroup.com\/api-chemical-development\/?page_id=50894"},"modified":"2025-11-19T09:30:37","modified_gmt":"2025-11-19T09:30:37","slug":"reference-standard-preparation-and-characterisation","status":"publish","type":"page","link":"https:\/\/www.almacgroup.com\/api-chemical-development\/reference-standard-preparation-and-characterisation\/","title":{"rendered":"Reference Standard Preparation and Characterisation"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\" id=\"h-using-spectroscopic-techniques-for-peptide-reference-standard-preparation-and-peptide-characterisation\">Using Spectroscopic Techniques for peptide reference standard preparation and peptide characterisation<\/h4>\n\n\n\n<p>A reference standard of the API can be prepared by drawing material from the GMP batch. A specification reference standard and Certificate of Analysis will be issued.<\/p>\n\n\n\n<p>The reference standard can be characterised in a number of ways, using a number of spectroscopic techniques:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Accurate mass determination of molecular weight by reflectron QTOF MS analysis (better than 1 ppm mass accuracy)<\/li><li>Peptide sequencing by QTOF \u2013 CID \u2013 MS\/MS<\/li><li>Ratio of amino acids by amino acid analysis (performed&nbsp;to an approved Almac subcontractor)<\/li><li>Chirality of amino acids to be determined by chiral LC-MS analysis after hydrolysis with DCl<\/li><li>Structural elucidation by 1D and 2D NMR (will require experiments such as COSY, TOCSY, HSQC, HMBC and ROESY)<\/li><\/ul>\n\n\n<div class=\"o-wrapper\">\n\t<div class=\"o-grid\">\n\n\t\t<div class=\"o-grid__item u-width-full u-align-center\">\n\t\t\t\t\t\t\t<h2 class=\"u-m-b4\">Related Resources<\/h2>\n\t\t\t\t\t<\/div>\n\n\t<\/div>\n            <div class=\"o-grid u-flex-align-stretch u-m-b3\">\n\n            \n                                            <article class=\"o-grid__item u-width-4\/12 u-width-4\/12@l u-width-6\/12@m u-width-full@s u-flex u-m-b3 u-a-full\">\n                    <div class=\"c-related-resources__panel u-flex u-flex-direction-column u-flex-align-center u-align-center u-p-x4 u-p-y3 u-m-b2\">\n                \n                                                <img decoding=\"async\" class=\"u-m-b2 lazyload\" data-src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/peptides-catalogue-scaled-1.jpeg\" alt=\"5 Dimensional Structural Characterisation of Synthetic Peptides\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 2560px; --smush-placeholder-aspect-ratio: 2560\/1714;\"><noscript><img decoding=\"async\" class=\"u-m-b2\" src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/peptides-catalogue-scaled-1.jpeg\" alt=\"5 Dimensional Structural Characterisation of Synthetic Peptides\"><\/noscript>\n                        \n                                                    <p class=\"c-resource__category\">Poster<\/p>\n                        \n                                                    <h4 class=\"u-m-t2\">5 Dimensional Structural Characterisation of Synthetic Peptides<\/h4>\n                        \n                        \n                                                                                                                        <a class=\"u-m-t-auto c-btn c-btn-secondary c-btn--none\" href=\"https:\/\/almacgroup.com\/knowledge\/library\/5-dimensional-structural-characterisation-of-synthetic-peptides\/\" target=\"\">View resource<\/a>\n                                                                                                        <\/div> <!-- \/c-related-resoucres__panel -->\n                    <\/article>\n                    <\/div>\n    <\/div>\n\n\n\t<article class=\"o-grid o-grid--full\">\n\n\t\t<div class=\"o-grid__item u-width-full u-m-t6 u-m-t4@m u-m-b4\">\n\t\t\t<h2 class=\"u-normal u-a-center\"><\/h2>\n\t\t<\/div>\n\n\t\t<div class=\"u-flex u-flex-justify-center u-flex-wrap u-width-full c-news-panels__slider js-news-panels\">\n\t\t\t\t\t<\/div>\n\t<\/article>\n\n\n<!-- <article id=\"related\">\n\t<div class=\"o-grid\">\n\t\t\n\t\t<div class=\"u-flex u-flex-justify-center u-flex-wrap u-width-full c-resources__slider js-resources-panels\">\n\t\t\t\n\t\t\t\t\t<\/div>\n\t<\/div>\n<\/article> -->\n\n\n\n<div style=\"height:68px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n\n\n\n\n<p><a class=\"c-btn c-btn--red\" href=\"https:\/\/www.almacgroup.com\/api-chemical-development\/contact-us\/\">Contact us<\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Using Spectroscopic Techniques for peptide reference standard preparation and peptide characterisation A reference standard of the API can be prepared by drawing material from the GMP batch. A specification reference standard and Certificate of Analysis will be issued. The reference standard can be characterised in a number of ways, using a number of spectroscopic techniques: [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-50894","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v25.3.1 (Yoast SEO v26.3) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Reference Standard Preparation and Characterisation - API Services &amp; Chemical Development<\/title>\n<meta name=\"description\" content=\"The reference standard of the API can be characterised in a number of ways, using a number of spectroscopic techniques.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.almacgroup.com\/api-chemical-development\/reference-standard-preparation-and-characterisation\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Reference Standard Preparation and Characterisation\" \/>\n<meta property=\"og:description\" content=\"The reference standard of the API can be characterised in a number of ways, using a number of spectroscopic techniques.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.almacgroup.com\/api-chemical-development\/reference-standard-preparation-and-characterisation\/\" \/>\n<meta property=\"og:site_name\" content=\"API Services &amp; 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