{"id":52141,"date":"2021-08-02T15:02:11","date_gmt":"2021-08-02T14:02:11","guid":{"rendered":"https:\/\/www.almacgroup.com\/api-chemical-development\/?page_id=52141"},"modified":"2025-03-11T15:06:33","modified_gmt":"2025-03-11T15:06:33","slug":"synthesis-of-single-stranded-rna-oligonucleotides","status":"publish","type":"page","link":"https:\/\/www.almacgroup.com\/api-chemical-development\/synthesis-of-single-stranded-rna-oligonucleotides\/","title":{"rendered":"Synthesis of single stranded RNA oligonucleotides"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\" id=\"h-addition-of-single-nucleotides-to-form-blockmers\">Addition of single nucleotides to form blockmers<\/h2>\n\n\n\n<p>Using state-of the-art synthetic biology design, Almac has designed panels of diverse RNA ligases (RNAL) to screen reactions for coupling of a single nucleotide to an oligonucleotide (at least 3 residues in length).&nbsp;<\/p>\n\n\n\n<div class=\"wp-block-image u-p-y2\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" width=\"398\" height=\"239\" data-src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Single-stranded-RNA-ligases.png\" alt=\"\" class=\"wp-image-52142 lazyload\" data-srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Single-stranded-RNA-ligases.png 398w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Single-stranded-RNA-ligases-300x180.png 300w\" data-sizes=\"(max-width: 398px) 100vw, 398px\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 398px; --smush-placeholder-aspect-ratio: 398\/239;\" \/><noscript><img decoding=\"async\" width=\"398\" height=\"239\" src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Single-stranded-RNA-ligases.png\" alt=\"\" class=\"wp-image-52142\" srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Single-stranded-RNA-ligases.png 398w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Single-stranded-RNA-ligases-300x180.png 300w\" sizes=\"(max-width: 398px) 100vw, 398px\" \/><\/noscript><\/figure><\/div>\n\n\n\n<p>To continue extending the oligonucleotide another enzyme class, alkaline phosphatase (ALKP), can be used to cleave the 3\u2019 phosphate, providing a 5\u2019-hydroxyl which can be used for further RNA ligase extension if desired.<\/p>\n\n\n\n<div class=\"wp-block-image u-p-y2\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" width=\"331\" height=\"239\" data-src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Alkaline-Phosphatases.png\" alt=\"\" class=\"wp-image-52143 lazyload\" data-srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Alkaline-Phosphatases.png 331w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Alkaline-Phosphatases-300x217.png 300w\" data-sizes=\"(max-width: 331px) 100vw, 331px\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 331px; --smush-placeholder-aspect-ratio: 331\/239;\" \/><noscript><img decoding=\"async\" width=\"331\" height=\"239\" src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Alkaline-Phosphatases.png\" alt=\"\" class=\"wp-image-52143\" srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Alkaline-Phosphatases.png 331w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Alkaline-Phosphatases-300x217.png 300w\" sizes=\"(max-width: 331px) 100vw, 331px\" \/><\/noscript><\/figure><\/div>\n\n\n\n<p>Once the required blockmers are assembled larger strand construction can begin. The RNAL can couple both natural and unnatural nucleotides that have modifications in 2\u2019 position such as o-methyl or fluoro groups as well as incorporation of phosphorothioate linkages.<\/p>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-using-blockmers-to-build-single-stranded-rna-oligonucleotides\">Using blockmers to build single stranded RNA oligonucleotides<\/h2>\n\n\n\n<p>Almac use a template independent approach to link blockmers using our selectAZyme\u2122 RNAL enzyme panel. The enzymes can link fragments that have natural or 2\u2019 modified nucleotides as well as accommodating blockmers with phosphorothioate linkages.&nbsp;<\/p>\n\n\n\n<div class=\"wp-block-image u-p-y2\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" width=\"666\" height=\"307\" data-src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Using-blockmers-to-build-single-stranded-RNA-oligonucleotides.png\" alt=\"\" class=\"wp-image-52144 lazyload\" data-srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Using-blockmers-to-build-single-stranded-RNA-oligonucleotides.png 666w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Using-blockmers-to-build-single-stranded-RNA-oligonucleotides-300x138.png 300w\" data-sizes=\"(max-width: 666px) 100vw, 666px\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 666px; --smush-placeholder-aspect-ratio: 666\/307;\" \/><noscript><img decoding=\"async\" width=\"666\" height=\"307\" src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Using-blockmers-to-build-single-stranded-RNA-oligonucleotides.png\" alt=\"\" class=\"wp-image-52144\" srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Using-blockmers-to-build-single-stranded-RNA-oligonucleotides.png 666w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Using-blockmers-to-build-single-stranded-RNA-oligonucleotides-300x138.png 300w\" sizes=\"(max-width: 666px) 100vw, 666px\" \/><\/noscript><\/figure><\/div>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-using-double-stranded-rnal-synthesis-to-produced-single-stranded-rna-oligonucleotides\">Using double stranded RNAL synthesis to produced single stranded RNA oligonucleotides<\/h2>\n\n\n\n<p>Various strategies for constructing the oligonucleotide can be investigated through the use of Almac\u2019s double stranded RNAL within the selectAZyme\u2122 panel.&nbsp; A short complementary bridging sequence can be used to bring two single strands together for ligation with double stranded RNAL potentially providing more favourable conditions for ligation.<\/p>\n\n\n\n<div class=\"wp-block-image u-p-y2\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" width=\"1024\" height=\"257\" data-src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1.png\" alt=\"\" class=\"wp-image-52145 lazyload\" data-srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1.png 1024w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1-300x75.png 300w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1-768x193.png 768w\" data-sizes=\"(max-width: 1024px) 100vw, 1024px\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1024px; --smush-placeholder-aspect-ratio: 1024\/257;\" \/><noscript><img decoding=\"async\" width=\"1024\" height=\"257\" src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1.png\" alt=\"\" class=\"wp-image-52145\" srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1.png 1024w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1-300x75.png 300w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/Denature-1024x257-1-768x193.png 768w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/noscript><\/figure><\/div>\n\n\n\n<div class=\"u-a-left\"><a class=\"u-m-y4 c-btn c-btn-primary c-btn--red\" href=\"\/api-chemical-development\/contact-us\/\">Contact Us<\/a><\/div>\n\n\n<!-- TX1 title-image.php -->\n\t\t\t<div class=\"c-box-link-title-image inside bottom black strip-none standard centre\">\n\t\t\t<div class=\"o-wrapper\">\n\t\t\t\t<div class=\"o-grid u-flex-align-stretch\">\n\t\t\t\t\t<div class=\"o-grid__item u-width-full\">\n\t\t\t\t\t\t<h2 class=\"u-p-b4 u-a-center\">Biocatalytic Oligonucleotide Synthesis Technology<\/h2>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"o-grid u-flex-align-stretch u-m-b6@m u-overflow-hidden u-overflow-initial@l \">\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<article class=\"o-grid__item u-width-6\/12 u-width-full@s\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<!-- Show the correct button based off pop up to show or not -->\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/api-chemical-development\/synthesis-of-double-stranded-oligonucleotides\/\">\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<header class=\"c-link-box__image lazyload\" \n                                                                        role=\"img\" aria-label=\"\" \n                                                                        style=\"background-image:inherit;\" data-bg-image=\"url(&#039;https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/biotcatalytic.jpeg&#039;)\">\n   \t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/header>\n\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"c-link-box__content strip-none\">\n\t\t\t\t\t\t\t\t\t\t<div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"u-m-b2\">Synthesis of double stranded oligonucleotides<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"u-m-b2\">\n\t\t\t\t\t\t\t\t\t\t\t\tTo create a double stranded oligonucleotide a range of short complementary RNA sequences\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\n\n    <div class=\"  u-m-t-auto \">\n                            <div class=\"c-btn c-btn-secondary c-btn--none\">Find out more<\/div>\n                    <\/div>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t<!-- Only show pop up if acf is checked to show it -->\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/article>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<article class=\"o-grid__item u-width-6\/12 u-width-full@s\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<!-- Show the correct button based off pop up to show or not -->\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/api-chemical-development\/biocatalytic-oligonucleotide-synthesis-technology\/enzyme-panels-available-to-support-oligonucleotide-synthesis\/\">\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<header class=\"c-link-box__image lazyload\" \n                                                                        role=\"img\" aria-label=\"\" \n                                                                        style=\"background-image:inherit;\" data-bg-image=\"url(&#039;https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2021\/08\/4col-img-title.jpeg&#039;)\">\n   \t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/header>\n\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"c-link-box__content strip-none\">\n\t\t\t\t\t\t\t\t\t\t<div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h3 class=\"u-m-b2\">Enzyme panels available to support Oligonucleotide synthesis<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"u-m-b2\">\n\t\t\t\t\t\t\t\t\t\t\t\tLearn more about the Enzyme panels available to support Oligonucleotide synthesis\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\n\n    <div class=\"  u-m-t-auto \">\n                            <div class=\"c-btn c-btn-secondary c-btn--none\">Find out more<\/div>\n                    <\/div>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t<!-- Only show pop up if acf is checked to show it -->\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/article>\n\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t","protected":false},"excerpt":{"rendered":"<p>Addition of single nucleotides to form blockmers Using state-of the-art synthetic biology design, Almac has designed panels of diverse RNA ligases (RNAL) to screen reactions for coupling of a single nucleotide to an oligonucleotide (at least 3 residues in length).&nbsp; To continue extending the oligonucleotide another enzyme class, alkaline phosphatase (ALKP), can be used to [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-52141","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v25.3.1 (Yoast SEO v26.3) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Synthesis of single stranded RNA oligonucleotides - API Services &amp; Chemical Development<\/title>\n<meta name=\"description\" content=\"Almac has designed panels of diverse RNA ligases (RNAL) to screen reactions for coupling of a single nucleotide to an oligonucleotide.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link 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