{"id":53346,"date":"2024-03-21T16:20:28","date_gmt":"2024-03-21T16:20:28","guid":{"rendered":"https:\/\/www.almacgroup.com\/api-chemical-development\/?page_id=53346"},"modified":"2025-03-11T15:10:32","modified_gmt":"2025-03-11T15:10:32","slug":"smart-site-saturation-mutagenesis","status":"publish","type":"page","link":"https:\/\/www.almacgroup.com\/api-chemical-development\/smart-site-saturation-mutagenesis\/","title":{"rendered":"Smart Site Saturation Mutagenesis"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\" id=\"h-3-4-months-5-000-10-000-mutants\"><strong><strong>(3 \u2013 4 months 5,000 \u2013 10,000 mutants)<\/strong><\/strong><\/h3>\n\n\n\n<p>Molecular modelling, molecular docking, molecular dynamics, quantum mechanics and bioinformatic tools are used to sample the sequence space of the enzyme with the goal of identifying the enzyme hotspots, which are targeted for site saturation mutagenesis. All mutants are cloned, expressed and screened on an amino acid site by site basis for improvements in the reaction of interest.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"1296\" height=\"3536\" data-src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis.png\" alt=\"\" class=\"wp-image-53368 lazyload\" style=\"--smush-placeholder-width: 1296px; --smush-placeholder-aspect-ratio: 1296\/3536;width:840px;height:auto\" data-srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis.png 1296w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-110x300.png 110w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-375x1024.png 375w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-768x2095.png 768w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-563x1536.png 563w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-751x2048.png 751w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-205x560.png 205w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-117x320.png 117w\" data-sizes=\"(max-width: 1296px) 100vw, 1296px\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" \/><noscript><img decoding=\"async\" width=\"1296\" height=\"3536\" src=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis.png\" alt=\"\" class=\"wp-image-53368\" style=\"width:840px;height:auto\" srcset=\"https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis.png 1296w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-110x300.png 110w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-375x1024.png 375w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-768x2095.png 768w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-563x1536.png 563w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-751x2048.png 751w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-205x560.png 205w, https:\/\/www.almacgroup.com\/api-chemical-development\/wp-content\/uploads\/sites\/4\/2024\/03\/smart_site_saturation_mutagenesis-117x320.png 117w\" sizes=\"(max-width: 1296px) 100vw, 1296px\" \/><\/noscript><\/figure>\n\n\n\n<style>#j-content > .o-grid {\n    display: flex;\n    flex-direction: row-reverse;\n}<\/style>\n","protected":false},"excerpt":{"rendered":"<p>(3 \u2013 4 months 5,000 \u2013 10,000 mutants) Molecular modelling, molecular docking, molecular dynamics, quantum mechanics and bioinformatic tools are used to sample the sequence space of the enzyme with the goal of identifying the enzyme hotspots, which are targeted for site saturation mutagenesis. All mutants are cloned, expressed and screened on an amino acid [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-53346","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v25.3.1 (Yoast SEO v26.3) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Smart Site Saturation Mutagenesis - API Services &amp; Chemical Development<\/title>\n<meta name=\"description\" content=\"Sample the sequence space of the enzyme with the goal of identifying the enzyme hotspots, which are targeted for site saturation mutagenesis.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.almacgroup.com\/api-chemical-development\/smart-site-saturation-mutagenesis\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Smart Site Saturation Mutagenesis\" \/>\n<meta property=\"og:description\" content=\"Sample the sequence space of the enzyme with the goal of identifying the enzyme hotspots, which are targeted for site saturation mutagenesis.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.almacgroup.com\/api-chemical-development\/smart-site-saturation-mutagenesis\/\" \/>\n<meta property=\"og:site_name\" content=\"API Services &amp; 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