Single cell 3’ RNA Sequencing Service

Single Cell 3’ RNA Sequencing Service

A Next Generation Sequencing (NGS) solution which uses the 10x Genomics Chromium X platform and Next GEM Single Cell 3’ workflow to generate RNA-seq data at the cellular level from cell suspension stored in Cryo-preservative.

The service includes a scalable bioinformatic pipeline underpinned by 10x Genomics Cell Ranger software enabling comprehensive QC and single cell gene expression estimation

Optional custom bioinformatics solutions are also offered such as cell type identification, differential expression analysis between cell types and pathway analysis.

For Research Use Only

Key benefits:

• Raw Sequence data delivered in FASTQ format, compatible with most bioinformatics pipelines for processing and interpreting single cell RNA Seq data.
• Interactive QC report enables rapid and comprehensive assessment of sequencing, mapping, and cell metrics.
• Optional bioinformatics services including fully customized downstream analysis and reporting.

Almac 3’ Single Cell Seq RNA Sequencing Pipeline: Data Flow

Almac 3’ scRNA SEQ: Outputs

• Raw sequence in FASTQ

• Scalable 3’ scRNA SEQ pipeline hosted on Illumina connected Analytics (ICA) cloud platform enabling:

• Pre & post sequence alignment QC (standard)

  • Sample & sequence/alignment Microsoft excel QC report
  • Cellranger interactive summary HTML file containing QC metrics and automated secondary analysis results including t-SNE projection of cells.

• Additional Outputs:

  • UMI Count Matrix: Equivalent to Raw Count Matrix but counts the number of UMIs per gene for each cellular barcode.
  • Cloupe File: Input to Cellranger Loupe Browser for efficient data exploration and differential expression analysis.

Interested in Almac’s Single cell 3’ RNA Sequencing Service? Submit your details below: