Whole Genome Sequencing (WGS) Service
Whole Genome Sequencing (WGS) Service
A high quality, robust Next Generation Sequencing (NGS) solution built on Roche’s platform. Almac’s wet lab workflow generates comprehensive WGS data from blood and FFPE.
The platform covers coding and non-coding regions with readily interpretable raw sequencing data for use in biomarker discovery and retrospective clinical investigation.
QC guarantee of 30X / 80X mean target coverage across sample cohort ensures comprehensive downstream analysis and data interpretation.
For Research Use Only
Robust Almac WGS Data Generation Protocol
Technical Specification and Performance Validation:
Almac analytical validation data confirms the highly accurate, repeatable and reproducible performance of the WGS service.
For full technical specification & further validation information download the fact sheet.
Benefits:
- QC guarantee of 30X / 80X mean target coverage across sample cohort ensures comprehensive downstream analysis and data interpretation
- Protocol optimised for blood and FFPE samples
- Interactive QC report enables rapid and comprehensive sample quality interrogation across the cohort
- Raw Sequence data delivered in FASTQ format, compatible with the majority of bioinformatics pipelines
- Germline / Somatic variant discovery service: filtered and annotated germline variants delivered as standard VCF files compatible with majority of downstream interpretation tools
Outputs:
- Raw sequence in FASTQ format
- Interactive HTML sequence/alignment QC report
- Germline / Somatic variant calls delivered as VCF files compatible with majority of downstream interpretation tools- Raw and Filtered + annotated
- Custom downstream variant analysis options are also offered.