{"id":1125,"date":"2020-03-17T13:43:48","date_gmt":"2020-03-17T13:43:48","guid":{"rendered":"https:\/\/www.almacgroup.com\/diagnostics\/?post_type=diagnostic_resource&#038;p=1125"},"modified":"2020-03-17T13:43:48","modified_gmt":"2020-03-17T13:43:48","slug":"development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue","status":"publish","type":"diagnostic_resource","link":"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/","title":{"rendered":"Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue"},"content":{"rendered":"\n<p>There is considerable interest in the use of gene expression data to generate predictive and prognostic biomarkers from archived non-small cell lung cancer (NSCLC) tissue (Potti et al., 2006). In early stage disease, up to twenty FFPE blocks are available from each patient. Since NSCLC tumors exhibit histological diversity, it is possible that areas of individual tumors represented by different FFPE blocks will exhibit distinct molecular profiles (Sakurada et al., 2008). This intratumoral&nbsp; heterogeneity represents a potential problem for the development of biomarkers from NSCLC tissue. This study examines gene expression data in multiple FFPE blocks taken from individual patients. Variations in gene expression are analysed between:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>FFPE blocks of individual patients<\/li><li>Whole FFPE sections and macrodissected tumor tissue<\/li><li>Individual patients<\/li><li>Disease histologies (squamous and adenocarcinoma<\/li><\/ul>\n\n\n\n<p>The study also evaluates the impact of these variations on powering clinical biomarker discovery studies.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.almacgroup.com\/wp-content\/uploads\/2015\/01\/Development-of-Expression-Based-Biomarkers-in-NSCLC-A-study-of-Intratumor-Heterogeneity-Using-FFPE-Tissue-20101.pdf\">Click here to read more<\/a><\/p>\n","protected":false},"featured_media":0,"template":"","diagnostics_type":[476],"class_list":["post-1125","diagnostic_resource","type-diagnostic_resource","status-publish","hentry","diagnostics_type-diagnostics-posters"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v25.3.1 (Yoast SEO v26.3) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue - Diagnostic Services<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue\" \/>\n<meta property=\"og:description\" content=\"There is considerable interest in the use of gene expression data to generate predictive and prognostic biomarkers from archived non-small cell lung cancer (NSCLC) tissue (Potti et al., 2006). In early stage disease, up to twenty FFPE blocks are available from each patient. Since NSCLC tumors exhibit histological diversity, it is possible that areas of [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/\" \/>\n<meta property=\"og:site_name\" content=\"Diagnostic Services\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.almacgroup.com\/diagnostics\/wp-content\/uploads\/sites\/3\/2020\/11\/Almac_Logo-e1698941154737.jpeg\" \/>\n\t<meta property=\"og:image:width\" content=\"100\" \/>\n\t<meta property=\"og:image:height\" content=\"52\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/\",\"url\":\"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/\",\"name\":\"Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue - Diagnostic Services\",\"isPartOf\":{\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/#website\"},\"datePublished\":\"2020-03-17T13:43:48+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.almacgroup.com\/diagnostics\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/#website\",\"url\":\"https:\/\/www.almacgroup.com\/diagnostics\/\",\"name\":\"Diagnostic Services\",\"description\":\"Just another Almac Core Sites site\",\"publisher\":{\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.almacgroup.com\/diagnostics\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/#organization\",\"name\":\"Almac Group Ltd\",\"url\":\"https:\/\/www.almacgroup.com\/diagnostics\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.almacgroup.com\/diagnostics\/wp-content\/uploads\/sites\/3\/2020\/04\/almac-logo-with-strapline.jpg\",\"contentUrl\":\"https:\/\/www.almacgroup.com\/diagnostics\/wp-content\/uploads\/sites\/3\/2020\/04\/almac-logo-with-strapline.jpg\",\"width\":643,\"height\":296,\"caption\":\"Almac Group Ltd\"},\"image\":{\"@id\":\"https:\/\/www.almacgroup.com\/diagnostics\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue - Diagnostic Services","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.almacgroup.com\/diagnostics\/diagnostic-resource\/development-of-expression-based-biomarkers-in-nsclc-a-study-of-intratumor-heterogeneity-using-ffpe-tissue\/","og_locale":"en_GB","og_type":"article","og_title":"Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue","og_description":"There is considerable interest in the use of gene expression data to generate predictive and prognostic biomarkers from archived non-small cell lung cancer (NSCLC) tissue (Potti et al., 2006). 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