Resources by service
Mass Spectrometry: 5 Dimensional Structural Characterisation of Synthetic Peptides
There is currently growing interest in the therapeutic benefits of synthetic peptides. These molecules, like small molecule pharmaceuticals, require comprehensive characterisation to unequivocally confirm the structure as per the USP guidelines. Full identity elucidation requires integration of orthogonal techniques to overcome the limitations associated with each technique. Presented is an example of the characterisation protocol developed at Almac for synthetic peptides.
This process confirms peptide sequence by Nuclear Magnetic Resonance and Tandem Mass Spectrometry, peptide molecular formula by accurate mass determination, chiral purity by pre-column derivatisation of the peptide hydrolysates followed by liquid chromatography with mass spectrometry detection as well as ratio determination of each amino acid in the sequence. This characterisation protocol goes beyond the requirements of the USP and gives confidence in the identification of synthetic peptides. The protocol has already been used for peptides containing up to 34 amino acids.Download Contact us