Accurate determination of resin substitution in solid phase peptide synthesis
Accurate resin substitution is critical in solid phase peptide synthesis, influencing reagent requirements, process yields, product quality and overall manufacturing efficiency. This case study explores a robust approach developed by Almac Sciences for determining substitution of highly moisture-sensitive 2-chlorotrityl chloride (2-CTC) resin. Combining resin loading and cleavage with UHPLC quantification, the validated three-step method helps overcome moisture-related degradation challenges and provides accurate, precise results suitable for routine analysis and release purposes.
Read the case study to discover how a robust UHPLC-based approach can support accurate resin substitution determination in solid phase peptide synthesis.