Fusion enzymes represent a powerful innovation in biocatalysis, combining multiple enzymatic functions into a single, efficient catalyst. One such example is the fusion which merges phosphite dehydrogenase (PTDH) with Baeyer-Villiger monooxygenase (BVMO).
Advantages of Fusion Enzymes:
Only one catalyst is required, simplifying the reaction setup.
Soluble expression of tricky enzymes can be improved by a more soluble N-terminal fusion partner.
Thermostability may be enhanced through a thermostable fusion partner.
Increased efficiency in mass transfer due to closer proximity of cofactor-producing and cofactor-using enzymes.
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