Use of SDS-PAGE for Residual Protein Determination
Biocatalytic API manufacturing can introduce residual host cell proteins and recombinant enzymes that need to be effectively detected and controlled. This white paper explores how Almac Sciences uses SDS-PAGE as a robust and versatile approach for residual protein determination, offering both qualitative and semi-quantitative assessment across complex API matrices. It demonstrates how the method can support routine quality control, process optimisation and in-process enzyme monitoring while overcoming some of the limitations associated with traditional protein analysis techniques.
Read the white paper to discover how SDS-PAGE can support robust residual protein determination and quality control in biocatalytic API manufacturing.