Technology compatible with prokaryotic and eukaryotic expression

Technology compatible with prokaryotic and eukaryotic expression

 

Expression in Echerichia coli

  • Cytoplasmatic expression of Evasin 3- intein in bacteria
  • Intein cleavage followed by site specific C-terminal PEGylation of target protein
  • Technology also compatible with periplasmic expression of the intein-fusion proteins

Expression in Pichia pastoris

  • Secreted expression of EGFR sdAb-intein from yeast
  • Intein cleavage followed by site specific C-terminal PEGylation of target protein 
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