Enzyme panels available to support Oligonucleotide synthesis

• Diverse panel of enzymes that contain thermophilic homologues that work up to 65-70 °C
• Substrate preference single stranded RNA
• Enzymes can accept unnatural 2’ modifications of 3’,5’-diphosphate donors
• Single nucleoside addition
• Coupling of oligomers
• Panel of available 96 enzymes

• Diverse panel of ligases from bacterial and viral sources
• Complementary base-pairing allows for blockmers to anneal to form duplex followed by ligation of adjacent blockmers by dsRNAL
• Panel of available 96 enzymes

• For the removal of terminal phosphate to allow further extension of oligonucleotide
• Highly thermostable and can be heat purified
• Panel of available 96 enzymes

• PNPase catalyzes the reversible polymerization of ribonucleoside diphosphates to polyribonucleotide with the release of inorganic phosphate
• Panel of available 96 enzymes

• Catalyses the cleavage of the glycosidic bond of 2′-deoxyribonucleosides and the transfer of the deoxyribosyl moiety to an acceptor purine or pyrimidine base
• Useful for synthesis of modified nucleoside building blocks.
• Panel of available 50 enzymes

• Phosphorylases (PPL) are known to be more promiscuous than NDTs in 2’ position and are known to be active against nucleosides containing F, NH2, OH and H in 2’ position.
• Panel of 25 purine and 25 pyrimidine nucleotide phosphorylases

Polynucleotide kinase (PNK)

• Transfers phosphate from ATP to 5’OH of RNA blockmers.
• Panel of 50 enzymes available