Enzyme panels available to support Oligonucleotide synthesis

 
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Panels currently available

Single stranded RNA ligases

  • Diverse panel of enzymes that contain thermophilic homologues that work up to 65-70 °C
  • Substrate preference single stranded RNA
  • Enzymes can accept unnatural 2’ modifications of 3’,5’-diphosphate donors
  • Single nucleoside addition
  • Coupling of oligomers
  • Panel of available 96 enzymes

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Double stranded RNA ligases

  • Diverse panel of ligases from bacterial and viral sources
  • Complementary base-pairing allows for blockmers to anneal to form duplex followed by ligation of adjacent blockmers by dsRNAL
  • Panel of available 96 enzymes

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Alkaline phosphatases

  • For the removal of terminal phosphate to allow further extension of oligonucleotide
  • Highly thermostable and can be heat purified
  • Panel of available 96 enzymes

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Polynucleotide phosphorylases

  • PNPase catalyzes the reversible polymerization of ribonucleoside diphosphates to polyribonucleotide with the release of inorganic phosphate
  • Panel of available 96 enzymes

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Nucleoside deoxyribosyltransferases

  • Catalyzes the cleavage of the glycosidic bond of 2′-deoxyribonucleosides and the transfer of the deoxyribosyl moiety to an acceptor purine or pyrimidine base
  • Useful for synthesis of modified nucleoside building blocks.
  • Panel of available 50 enzymes

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Phosphorylases

  • Phosphorylases (PPL) are known to be more promiscuous than NDTs in 2’ position and are known to be active against nucleosides containing F, NH2, OH and H in 2’ position.
  • Panel of 25 purine and 25 pyrimidine nucleotide phosphorylases

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Polynucleotide kinases

Polynucleotide kinase (PNK)

  • Transfers phosphate from ATP to 5’OH of RNA blockmers.
  • Panel of 50 enzymes available

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Panels in development

  • DNA ligase 
  • Phosphodiesterase
  • Phosphotriesterase
  • Terminal deoxynucleotidyl transferase 
  • DNA polymerases
  • Phosphoramidases

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