Home / Knowledge Centre /

Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue

There is considerable interest in the use of gene expression data to generate predictive and prognostic biomarkers from archived non-small cell lung cancer (NSCLC) tissue (Potti et al., 2006).

In early stage disease, up to twenty FFPE blocks are available from each patient. Since NSCLC tumors exhibit histological diversity, it is possible that areas of individual tumors represented by different FFPE blocks will exhibit distinct molecular profiles (Sakurada et al., 2008). This intratumoral  heterogeneity represents a potential problem for the development of biomarkers from NSCLC tissue.

This study examines gene expression data in multiple FFPE blocks taken from individual patients. Variations in gene expression are analysed between:

  • FFPE blocks of individual patients
  • Whole FFPE sections and macrodissected tumor tissue
  • Individual patients
  • Disease histologies (squamous and adenocarcinoma

The study also evaluates the impact of these variations on powering clinical biomarker discovery studies.

View Resource
Almac Logo

Diagnostic Services

Your global partner for biomarker discovery, development & commercialisation.

Genomic Services

We offer a range of genomics platform and chemistry options, alongside expert data sciences support and a full sample management service.

Clinical Trial Assays

Comprehensive development, validation & delivery service for research and clinical use assays from our global clinical laboratories in USA, Europe and APAC.

Companion Diagnostics

A collaborative Companion Diagnostic partnership approach with a deep understanding of client needs. We offer CDx development, manufacture & commercialisation services.

Learn More

Download here instantly

This website uses cookies. By continuing to browse the site, you are agreeing to our use of cookies