Reference Standard Preparation and Characterization

 
Home /

Reference Standard Preparation and Characterization

Using Spectroscopic Techniques for peptide reference standard preparation and peptide characterization

A reference standard of the API can be prepared by drawing material from the GMP batch. A specification reference standard and Certificate of Analysis will be issued.

The reference standard can be characterized in a number of ways, using a number of spectroscopic techniques:

  • Accurate mass determination of molecular weight by reflectron QTOF MS analysis (better than 1 ppm mass accuracy)
  • Peptide sequencing by QTOF – CID – MS/MS
  • Ratio of amino acids by amino acid analysis (performed to an approved Almac subcontractor)
  • Chirality of amino acids to be determined by chiral LC-MS analysis after hydrolysis with DCl
  • Structural elucidation by 1D and 2D NMR (will require experiments such as COZY, TOCSY, HSQC, HMBC and ROESY)

Contact us

This website uses cookies. By continuing to browse the site, you are agreeing to our use of cookies