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Identification and Structural Elucidation by Amino Acid Sequencing of Peptide Impurities by 2DlC-MS/MS

Peptide impurity profiles require increasingly complex separation methods in order to detect and adequately quantitate peptide impurities. Mobile phase conditions optimal for these separations in most cases consist of non-volatile salt based buffer systems that are not inherently LC/MS compatible. 2D-LC has been demonstrated to overcome this issue by extracting the impurity peaks of interest and transferring them to a mass spectrometry compatible solvent system for further analysis.
This webinar demonstrates that a 2D-LC system when paired with a Q-TOF mass spectrometer can automate impurity extraction by 2D-LC to facilitate identification by 2D-LC/MS and also further structural elucidation by 2D-LC MS/MS.

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Almac API, Chemical Development, Analytical & Solid State Services

Almac Sciences provides integrated services from development to commercial scale of advanced intermediates and Active Pharmaceutical Ingredients (API).

We provide a range of services for small molecules (including highly potent) and peptides. We have a proven track record of saving time and cost through the integration of our services and application of innovative biocatalysis and technology solutions.

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We offer a full suite of analytical testing for a range of different product types including small molecules, peptides, biologics, conjugates, potent and controlled substances.

Our vast pool of scientific knowledge can help to overcome the analytical challenges that typically arise during drug development.

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